Parental Estrangement Essays - Family, Parenting, Human Development

Parental Estrangement Estranged children The difference between an estranged child and an alienated child is that an estranged child has grown apart from the parent for reasons that are, to be blunt, reasonable, and realistic. An estranged child is either absolutely ambivalent about the other parent or enraged by the other parent. These feelings are, however, justified by the child's experience of the separation or by the child's experience of that parent. A parent who leaves the family home, enters a new relationship, and neglects time with their children and dismisses the harm done to their children is likely to become "estranged" from them. It is fair to say that no one responds positively to poor treatment, least of all children. Estrangement results from a parent behaving badly toward his/her children which, in return causes the children to cut off contact. It isn't uncommon for a parent who is estranged from his/her children to blame the other parent of parental alienation. It is easier to blame others for bad behaviour than to accept and acknowledge bad behaviour. When estrangement occurs, the justification is usually very understandable, troubling, and valid. The departing grown child often has been very badly psychologically emotionally damaged in the relationship. Here are some of the most common root problems why parental estrangement occurs. Divorce: Following a divorce, some children reject one parent, limit contact, or show extreme reluctance to be with the parent. Remarriage: Parent's remarriage may also cause tension. It can provoke or exacerbate unwarranted rejection. Lack of routine: If there is no routine or schedule in place the child may feel anxious, children need routines to help them feel safe. Personality Disorders: Many parents are difficult or overbearing, but some parents are too toxic to be around especially narcissist parents. They don't see their own behaviour as playing a role in the problem; they feel entitled to behave badly with no repercussions. Intolerance: children are going to make decisions that parents may not necessarily agree with. Parental estrangement occurs when parents fail to understand their children, never willing to look at situation from their children's point of view. Constant humiliation before others: Parents unaware/intentionally made their children to feel that s/he is good for nothing'; there is no regard for his/her dignity and sentiments. Priorities and Time. These are people who go weeks at a time without contacting their children because they are wrapped up in their career life. They don't understand why their children aren't waiting with arms wide open when they do find time to fit them into their schedule. Unresolved conflicts. Unhealthy conflict management create a basis for conflict to occur again. Recurring family arguments during significant holidays could deteriorate parents-child relationships leading to minimal contacts in the future. Witnessing violence committed by that parent against the other parent Being the victim of abuse from that parent The parent's persistently immature and self-centred behaviour The parent's unduly rigid and restrictive parenting style The parent's own psychological or psychiatric issues. Behaviours Common to an Estranged Parent: The parent who is estranged from a child due to his/her own bad treatment of the child has a "wait and see" attitude. They don't pursue a relationship with the child because in their mind the child is the one responsible for mending the relationship. The estranged parent will find it hard or impossible to view the situation from their child's perspective. They don't see their own behaviour as playing a role in the problem; they feel entitled to behave badly with no repercussions. These parents won't commit to a routine to see their child, they fit the child in when they have spare time rather fit their life around the child. These are people who go weeks at a time without contacting their children because they are wrapped up in a new relationship, focusing more on their career, spending time with the other man/woman or busy building a new life. They don't understand why their children aren't waiting with open arms when they do find time to fit them into their schedule. A checklist for parents when thinking about their estranged child: Have you asked your child what they honestly feel is the problem? Are you really listening to what my child is telling you? Is there a kernel of truth to any of what your child feels is wrong in your

Study Guide Exam 1 fin 317 Essay

Study Guide Exam 1 fin 317 Essay Study Guide Exam 1 fin 317 Essay Study Guide Exam 1: FIN 317 PPSet 1: ïÆ'˜ï€  List the four steps of the traditional risk management process. 1. Identify risk 2. Evaluate risk 3. Select a RM for each risk a. Risk avoidance b. Loss control c. Risk retention d. Risk transfer 4. Implement and review ïÆ'˜ï€  Understand Evolution of Enterprise Risk Management (ERM) from Traditional Risk Management (TRM) at a very simple level. Enterprise Risk Management - ERM - It considers all risks simultaneously and manages risk in a holistic or enterprise-wide (and risk-wide) context ïÆ'˜ï€  Where does insurance fit into the Risk Management Process? At the base of the financial pyramid ïÆ'˜ï€  Understand parts of the Personal Financial Planning Pyramid. Covers life, health , loss of income and property-liability income Wealth distribution wealth accumulation risk management (protection) Wealth accumulation: Speculation: investments like futures and commodities and real estate Growth and diversification: investments like bonds and stocks Savings and accumulation: regular savings and ownership of your house and education Risk management: Life, health, disability , long term care, property , liability insurance ïÆ'˜ï€  What professional exams is this course useful in preparing you for? Chartered Property Casualty Underwriter (CPCU) exams Associate in Risk Management (ARM) exams Certified Financial Planner (CFP) exams Chartered Life Underwriter (CLU) exams ïÆ'˜ï€  What is Gamma Iota Sigma (GIS)? An international business fraternity Promotes student interest in insurance, risk management, and actuarial science A place for students to gain leadership experience Prepares students for employment in the insurance and risk management professions. Provides networking opportunities for students to meet with various insurance and risk management professionals. PPSet 2: ïÆ'˜ï€  Risk Definitions Risk – uncertainty concerning loss, 2 types speculative and pure. ïÆ'˜ï€  Understand different classes of risk: pure, speculative, subjective, objective, diversifiable, and un-diversifiable Pure – Exists when there is uncertainty as to whether loss will occur Two possibilities: loss or no loss. Home and car, fire and flood risk Speculative – Exists when there is uncertainty about an event that can produce either a profit or a loss. investment risk, reputational risk, strategic risk Subjective – refers to the mental state of an individual who experiences doubt or worry as to the outcome of a given event It is essentially the psychological uncertainty that arises from an individual’s mental attitude or state of mind Objective – differs from subjective risk in the sense that it is more precisely observable and therefore measurable Often measured as probable variation around expected loss. Diversifiable – Un-Diversifiable - ïÆ'˜ï€  In general, what kinds of risk are insurable? Pure objective risks Risks covered: possibility that one of these perils may interrupt the individual’s income or cause medical expenses. Perils: death accidents and sickness living too long unemployment ïÆ'˜ï€  What are the Two Main Categories of Insurable Risks? Property can include all your land, house, apartment, contents (your stuff), car and so on. Liability is coverage for law suits you or your property may cause to others. When you insure your home this is how the property policy is layed out. Everything is a percentage of the dwelling or structure so what you have for a property limit on the structure affects everything else. Any you have deductibles you have to pay in a loss and the company wants you to insure close to the value and cost to rebuild that is co insurance usually about 80% or greater of the home’s cost to rebuild is required. Coverage A - Dwelling Coverage B – Other Structures 10% of A Coverage C – Contents 50% to 70% of A Coverage D – Loss of Use 10% of A Coverage E - Liability Coverage F – Medical payments Property liability risk - hazards such as wet roads, oily rags or things that might lead to a peril or loss

ET Essay Example | Topics and Well Written Essays - 1500 words

ET - Essay Example In this scenario, the link between BA and strategy is coordinated. Here the BA function is purely reactive and there is no feedback procedure from BA to strategy. Method competencies help a business analyst to keep the tool kit in order. He or she should be able to deliver multiple information in same data and should be able to visualize the information as the user. Answer. A data warehouse provides an organization with data which is consistent, integrated and valid. It ensures the collection of data from its source. Data warehouse retains the desired key features and provides data for creating reports. Answer. A company can collect source data either by collecting primary data or secondary data. The data collected for the first time is called the primary data. A primary data when further used becomes secondary data. A secondary data is collected from already collected data.The data sources for a company can be classified as: 1.Data generating source system- A data generating source system specifies which systems which create data for the first time and which don’t. Some examples of data generating source systems are billing systems, Reminder systems, Debt collection systems, CRM systems, and Product and consumption information. 2. Source system -When a data generated for the first time is saved, it becomes the source system for data warehouse. Data in the warehouse comes from primary sources and is saved on the basis of organizations rules and requirements. Based on the data warehouse information a large number of analysis and business processes are carried. 1. Establishment of information wheel- In order to provide the right information to the right people at the right time, data is condensed into information and knowledge. This is called information wheel. An information wheel summarizes the knowledge and information. 2. Creating Synergies between Information Wheels- The BICC must maintain the information wheels. The BICC

Case Study analysis for Marketing Communications Essay

Case Study analysis for Marketing Communications - Essay Example Companies in business areas such as beer and cider as well as other beverages benefit from sponsorship of sports personalities, clubs and events as these are opportunities for highlighting the spirit of entertainment and social celebrations, which is one of the intrinsic values of the product. The company is launching its advertising campaign on a rolling regional basis, starting from Southern London England followed by Scotland. However, when entering a market as UK, which is not very large in its size, it may be more effective to plan a national campaign, which will utilize national TV, and Press backed by outdoor advertising. The largest poster may be an effective means of attracting attention but the exposure level of the location is limited to those who are traveling via Heathrow airport to Ireland. The cost effectiveness per exposure may question the effectiveness of this advertising tool. The product positioning currently being conveyed by the campaign focuses on the product’s naturalness, tradition and heritage. Although these aspects appealed to the nationalistic Irish public, it may not be an effective positioning to the British. The company to adopt the â€Å"Pint Over Ice† concept combined with the naturalness aspect of the C&C’s product. The key message should be â€Å"Pint Over Ice† which targets the popularity of serving beverages in draught form in UK. The concept of â€Å"Pint On Ice† should be promoted as an integrated message through TV Ads, Press, Bill Boards as well as on location promotions with serving demonstrations. As the key challenge is to change the image of the cider product in UK public’s mind, the marketing communications focus has to be on above the line TV and press advertising conveying the premium image. The company should extend its advertising activities to pub level with Point of Sales material and promotions to add excitement. By providing branded product premiums such as caps, key tags

Womens role in Chinese film Movie Review Example | Topics and Well Written Essays - 1750 words

Womens role in Chinese film - Movie Review Example One such film is Ju Dou, which was originally produced in 1990 (Zeng 75). The film features typical Chinese culture and elements especially the ancient ones. While the film has multiple themes, one of the most evident themes is the role of women. The main character, Ju Dou, is a typical Chinese woman who brings out the roles that Chinese women played in the 1920s, which is time setting of the film (Singh 4). Apparently, Ju Dou plays multiple roles including being a wife, a mother, an aunt, and a widower. However, her character in the film reveals the underlying roles of women including positive and negative ones. One of the main roles of women in Ju Dou is being wives. As the film begins, Ju Dou is made wife to an old man, Tianqing’s uncle. Before her marriage, the old uncle had two other wives who had already died. This shows that women are presented as wives. The fact that the old uncle would marry each time the wife died emphasizes the role of the woman as the wife. However, it is important to note that the role of the woman as a wife as depicted in the film is quite diverse. First, the woman plays the role of giving birth. The old uncle makes it clear in the film that he bought Ju Dou purposely for her to give him a son. His previous wives had died without giving him a son. So, he was desperate to get another wife who would give him the son. This role of the woman as child bearer is also evident when people make fun of Tianqing to get a woman who would bear him a son during the celebration party of Ju Dou’s newborn son. When Ju Dou finally bears a son, she becomes a darling of many. The old uncle is so excited that he starts treating her well and even organizes a celebration for her. Second, women play the role of sex objects for men’s pleasure. Tianqing’s uncle actually makes it clear to Ju Dou that he bought her and therefore he has all the

Histamine Stimulated Small Intestine

Histamine Stimulated Small Intestine The longitudinal smooth muscle of the guinea-pig ileum small intestine contracts in response to acetylcholine. These contractions can be reduced by the application of adenosine and related compounds. The guinea-pig ileum is innervated by the enteric, sympathetic and parasympathetic divisions of the autonomic nervous system. The parasympathetic and enteric fibres release acetylcholine which acts on muscarinic receptors. The action of adenosine and its receptor antagonists can be assessed by comparing electrically induced contractions via electrical field stimulation and histamine induced contractions. Electrical field stimulation contractions cause presynaptic release of acetylcholine to produce contractions where as the histamine induced contractions cause postsynaptic contractile responses. Throughout this study adenosine and its receptor antagonist actions will be investigated and compared using electrical field stimulation and histamine. The contraction of the gut In gastrointestinal smooth muscles, researches show that there are two types of muscarinic receptors types that are present as targets to the neurotransmitter acetylcholine (Okamoto et al., 2002).Acetylcholine and its derivatives produce contractions by activating muscarinic receptors. The muscarinic receptors types are known as M2 and M3. Binding Studies have portrayed that the number of M2 receptors is greater than that of the M3 receptors however functional studies have shown that M3 muscarinic receptors play a fundamental role in mediating the contractile response (Eglen et al., 1996) and the functional role of M2 exists as unclear (Clague et al., 1985). The M3 receptor is coupled with G proteins, causing activation of phospholipase C and formation of inositol trisphosphate and diacylglycerol, which are expected to contribute in muscarinic receptor mediated smooth muscle contractions (Unno et al., 2005). They also mediate relaxation due to the release of nitric oxide from neighbo uring endothelial cells. M3 receptors in visceral smooth muscle contribute to the smooth muscle stimulating effect of muscarinic agonists. However the muscarinic receptor most abundant in the ileum is the M2 which cause an indirect contraction of the guinea-pig ileum by preventing the relaxing effect of drugs (Ehlert and Thomas, 1995). Both muscarinic receptor subtypes are activated by acetylcholine and produce a contractile response; however they vary in their transduction mechanisms and signalling pathways. Adenosine Adenosine has numerous diverse roles in normal physiology; such roles include promoting/maintaining sleep, regulating state of encouragement as well as local neuronal excitability and coupling cerebral blood flow to energy demand (Dunwiddie and Masino, 2001). It exists free in the cytosol of all cells and is transported in and out of all cells mainly using a membrane transporter(Rang et al., 2007). Under normal conditions, adenosine is formed intracellularly as well as extracellularly (Fredholm et al., 2001). ATP is stored in vesicles and released by exocytosis. It is also available in the cytosol of cells and is taken up and released via a specific membrane transporter. Released ATP and ADP are rapidly converted to adenosine by the action of tissue nucleotides. Studies have shown that there are pathways that contribute to adenosine formation, a) by the action of adenylate kinase and cystolic 5-nucleotidase, b) formation from hydrolysis of adenosine 3, 5 phosphate and c) formation by the action of S-adenosylhomocysteine (SAH) hydrolase. The pharmacological effects of adenosine include smooth muscle relaxation and inhibition of nerve activity, lipolysis and platelet aggregation(Daly et al., 1983). There is evidence that stimulation or inhibition is of adenylate cyclise is involved in adenosine action and therefore it has been concluded that adenosine is mediated by cyclic AMP. Based on its ability to inhibit cell function and thus minimise the metabolic requirements of cells, one of its functions may be as a protective agent released when tissue integrity is threatened. Adenosine exerts its physiological actions activation of a number of specific cell surface receptors. There are four different adenosine receptors known as A1, A2A, A2B, and A3. Some characteristics of these receptors are presented in Figure 1a. These subtypes have been distinguished on the basis of their agonist and antagonist selectivity. They belong to the G protein-coupled receptors. Mechanism of Adenosine action Adenosine A1 receptors are negatively coupled to the inhibition of adenylate cyclase, however they can act through other pathways such as stimulation of phospholipase C, activation of potassium channels and inhibition of N-type calcium channels (Zizzo et al., 2009). A1 receptors are coupled to Gi and G0 proteins and lead to inhibition of adenylate cyclase and consequently cause a decrease in cAMP (Ranjit, 2008). Adenosine A2A and A2B are coupled for activation of adenylate cyclise whereas A3 receptors have been shown to stimulate phospholipase C and D, to inhibit adenylate cyclase and to activate ATP sensitive potassium channel (Ralevic and Burnstock, 1998). Activation of these receptors require comparatively high amounts of adenosine. A2A and A2B receptors have a high and a low affinity for adenosine respectively. Receptor Subtype A1 A2A A2B A3 Transduction mechanism Inhibits adenylyl cyclase Activates adenylyl cyclase Activates adenylyl cyclase Inhibits adenylyl cyclase Primary distribution Brain (cortex, cerebellum, hippocampus). Dorsal horn of spinal cord. Eye, adrenal gland, atria Spleen, thymus, leukocytes, blood platelets. Straitopallidal GABAergic neurons, olfactory bulb Caecum, colon, bladder Testis, mast cells Tissue functions Antinociception, Hypothermia. Sedation, Sleep, Inhibition of lipolysis, Cardio and neuroprotection Reflex tachycardia, vasodilation, inhibition of platelet aggregation, sleep protection against ischemia Relaxation of vascular and intestinal smooth muscle, cytokine production, inhibition of cell proliferation Mast cell degranulation, coronary vasodilation and protection from reperfusion Selective antagonists DPCPX PSB 36 SCH 58261 PSB 1115 potassium salt MRS 3777 hemioxalate Figure 1a: Summary of adenosine receptors. Adenosine and the enteric functions of the Gut The enteric nervous system (ENS) consists of a compilation of neurons in the gastrointestinal nervous system which is capable of functioning independently of the central nervous system. It moderates motility, secretion, microcirculation, inflammatory and immune responses of the gastrointestinal tract (Altaf and Sood, 2008). The ENS is composed of extrinsic, which consists of parasympathetic and sympathetic divisions and the intrinsic component which encloses neurons. Intestinal functions results from an interaction between the ENS, smooth muscle and the mucosal/immune system. The network is regulated by several mediators; however there is consolidating evidence that adenosine is a significant regulating agent (Bueno, 2000) (Wood, 2004). Studies show that in the small intestine adenosine and adenosine derivatives where found to inhibit cholinergic transmission in guinea-pigs via a prejunctional action on neurotransmitter release (Gustafsson et al., 1978). The action of the A1 receptor s allowed mediation of the inhibitory action of adenosine in the cholinergic transmission(Shinozuka et al., 1985) of motor neurones innervating circular and longitudinal smooth muscle however A2A receptors have been reported to reduce the cholinergic motor responses(Gustafsson et al., 1985a; Gustafsson et al., 1985b). Histamine Histamine has a role as a primary transmitter or neuromodulator and it is widely distributed within mammalian tissues. (Izzo et al., 1998). Histamine is a vasoactive substance to be identified in the body which can rapidly metabolise and holds properties of being highly polar and not diffusing readily across cell membranes or the blood-brain barrier. It is stored in mast cells and basophils of blood and has two receptors known as H1 and H2. The release of histamine could cause changes in the cardiovascular system and induce anaphylactic shock. Histamine has been shown to induce gastric acid secretion through the H2 receptors linked to cyclic AMP production in oxyntic cells. Researches show that gastric cells of the guinea-pig may have a class of binding sites for histamine which shows no relationship to adenylate cyclase and the H2 receptor. Histamine creates a spasmogenic effect on the intestine that results from H1 receptor stimulation(Guy A. and Settipane, 1988-1989). There are three histamine subtypes known as H1, H2 AND H3 and all three have been identified to be present in the guinea pig small intestine. Studies show that H1 receptor subtypes mediate the contraction of the longitudinal muscle in the small intestine (Izzo et al., 1998). However researchers also state that the effect of histamine is predominantly due to the interaction with H1 receptors located on smooth muscle cells and moderately due to the interaction H2 receptors present on myenteric plexus interneurones (Bauer and Matusak, 1988). AIMS The aim is to confirm the prejunctional action of adenosine and examine whether adenosine has the additional ability to relax the smooth muscle directly. The project will use histamine to contract the smooth muscle and the objective is to find out whether adenosine can reduce these contractions and if so is the concentration range similar to that needed to inhibit the contractions to the electrical field-stimulation? It will also be investigated what adenosine receptor subtype is involved (A1, A2A, A2B, A3 identified using selective antagonists). METHOD Animals and preparation of tissue Dunkin Hartley guinea-pigs (250g +) of male sex that had previously been fed Harlan 2040, the guinea-pig diet and ad lib filtered tap water, were obtained from Harlan UK. They were group housed and provided with grade 6 woodchip and hay bedding. Their enrichment consisted of plastic and cardboard fun tunnels, plastic igloos and gnawing blocks. Furthermore they were kept at room temperatures of 19-23 °C and at room humidity of 45-65%. They were provided with 12 hours light and 12 hours of dark lighting. The guinea-pigs were stunned by a blow to the head and sacrificed by exsanguination. Two segments of 3cm length were removed from the distal part of the small intestine, for each tissue the ends were tied with cotton threads to the tissue holder and then suspended in 20ml organ baths containing Krebs solution (composition in mM: NaCl, 118; NaHCO3, 25; Glucose, 11; KCl, 4.7; CACl2, 2.5; KH2PO4, 1.18; MgSO4, 1.18). This was aerated with 95% O2 and 5% CO2 and maintained at 37 °C. The tissues were left for 30minutes to equilibrate under a resting tension of 1g before starting the stimulation. Experimental protocol The organ baths were equipped with parallel electrodes which allowed electrical field stimulation to be transmitted at a frequency of 0.1Hz, 40V, 0.5ms pulse duration. Contractions of the ileum were measured with isometric transducers (ADInstruments Force Transducers), amplified and recorded onto a data capture system (Lab Charts on the PC). The tissues were allowed to stabilise in the organ baths in order to reach steady contractions. Figure 1b represents the experimental set up. C:UsersHomeDocumentsDSC00308.JPG Figure 1b shows the laboratory designed set up for the experiment, with a set of two organ baths. There were several parts (a-e) to the experiments to be carried out on the ileum. n is the number of ileum used throughout the experiment n=18. The number of experiments carried out on the ileum was 56. Effect of Adenosine-the experiment consisted of stimulating the tissues continuously with electrical field stimulation and adding cumulative concentrations of adenosine (10-11M 10-4M) to the organ baths and the responses were recorded. Effect of Histamine- the tissues were stimulated with histamine, cumulative concentrations of histamine (10-11M 10-4M) were added to the organ bath and responses were measured. The concentration 110-6M gave the maximum response and a steady contraction, it was used to contract the tissue with adenosine. Effect of Adenosine in the presence of Histamine- the concentration of histamine that gave the maximum and steady response was added to organ bath. The tissue was permitted to stabilise in order to reach steady contractions. Once reached, cumulative concentrations of adenosine were added (10-8M 10-4M) in order to observe the responses of the ileum to adenosine in the presence of histamine. Effect of PSB36(10-8M) SCH58261(10-7M) to confirm identity of receptors being investigated, cumulative dose-response curves for induced contractions by histamine were observed for adenosine in the presence of selective antagonists, PSB36 and SCH58261. An experiment was also carried out, which involved electrical field stimulation to contract the tissue, adenosine was added in the presence of these antagonists. Effect of Atropine Atropine concentrations of 10-6M 10-7M were added to establish the effects of atropine on the electrically field stimulated ileum. For each drug that was being tested except atropine, the experiment was repeated at least six times. The tissue was washed out three times at least after an experiment was completed and was allowed to recover before another experiment was carried out. The electrical field stimulation was also switched off each time the tissue was washed out. Chemicals and drugs The drugs that were used consisted of Adenosine hemisulphate salt, Histamine diphosphate salt and Atropine sulphate salt which were all purchased from Sigma-Aldrich, Poole, UK. PSB36 and SCH58261 and DMSO (Dimethyl sulfoxide) were purchased from Tocris -Cookson, Bristol, UK. All drugs were dissolved in distilled water with exceptions of PSB36 AND SCH58261 which were diluted with DMSO.10ml of stock solution were made up in each case. Statistical Analysis All drug concentrations presented were final bath concentrations. The drug effects were expressed as twitch contraction (g). All data were given as means  ± S.E.M, where n represents the number of animals from which tissues were taken and on which observations were made. Inhibitory effects by adenosine in the field stimulated guinea pig ileum were measured and the responses of ileum twitch contraction were recorded for each concentration applied. This was repeated when using adenosine receptor antagonists. Adenosine responses were fitted onto concentration-response curve. Effects of histamine stimulated guinea pig ileum were also recorded. Adenosine responses and its receptor antagonist response were measured and plotted. 3.0 RESULTS a. b. Figure 2. The effect of adenosine concentrations in the guinea-pig ileum. Data are means  ±S.E.M and are expressed as an average of contractions (g). a. Representative traces showing inhibitory responses induced by adenosine. b. Concentration response curve for adenosine representing the average twitch response (g) when cumulative adenosine concentrations were added. Each point with bar represents the mean  ± S.E.M (n=6). Figure 3a: Original trace illustrating twitch response abolished by atropine 10-6M final bath concentration. b. c. Figure 3. Influences of atropine and potent and selective A1 adenosine receptor antagonist PSB36 10-7M AND 10-8M on guinea-pig ileum. b. Twitch responses of the guinea-pig ileum preparation to electrical field stimulation in the presence of PSB36 10-7M (n=6) and PSB36 10-8M (n=4), compared to twitch responses to adenosine alone. The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. c. Contractions of the guinea-pig ileum preparation stimulated by histamine in the presence of PSB36 10-7M (n=6) and PSB36 10-8M (n=4), compared to responses to adenosine when stimulated with histamine (n=6). The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. a. b. Figure 4. Summarises the effect of potent and selective A2A adenosine receptor antagonist SCH58261 10-7M on guinea-pig ileum. a. Twitch responses of the guinea-pig ileum preparation to electrical field stimulation in the presence of SCH58261 10-7M (n=6), compared to twitch responses to adenosine alone. The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. b. Contractions of the guinea-pig ileum preparation stimulated by histamine in the presence of SCH58261 10-7M (n=6), compared to responses to adenosine when stimulated with histamine (n=6). The experiments carried out were not paired. Each point with bar represents the mean  ± S.E.M. Figure5a. A concentration-response curve illustrating the effects of adenosine on the guinea-pig ileum when the tissue is stimulated with histamine. . Each point with bar represents the mean  ± S.E.M (n=6). Figure 5b. Responses of the guinea-pig ileum to cumulative concentrations of histamine. Each point with bar represents the mean  ± S.E.M (n=8). Electrical field stimulated guinea-pig ileum produced reproducible twitch responses to adenosine. Adenosine concentrations ranging from 10-8M final bath concentration caused concentration dependent inhibitory effects. Administration of increasing adenosine concentrations decreased electrically evoked acetylcholine release from the cholinergic nerve endings of the ileum (Figure2). Figure 2b clearly portrays that adenosine has no effect at low final bath concentrations of 10-11M. Atropine 10-6M final bath concentration abolished the twitch responses to electrical field stimulation confirming they were produced by cholinergic nerve stimulation (Figure3). In the presence of selective A1 adenosine receptor antagonist PSB36 10-7M and 10-8M concentration and electrical field stimulation, the twitch responses to adenosine decreased, when adenosine was applied at higher concentrations, the twitch responses became smaller as acetylcholine release was inhibited and completely prevented by PSB36. Figure 3a shows the curve shifted to the right when PSB36 10-8 was administered compared to the curve of adenosine. When PSB36 10-7 was applied there is a slight shift of the curve to the right compared to the adenosine curve. The effects of the antagonist PSB36 are seen significantly at 10-8M final bath concentration as the shift of the curve is greater. The highest response the ileum produced was as at adenosine concentration of 1 x 10-8M, the responses lowered slightly at 1 x 10-6M and consequently decreased rapidly at 1 x 10-5M final bath concentrations. Histamine induces contractions in the ileum. Addition of cumulative concentrations of adenosine in the presence of PSB36 10-7M produced small responses compared to the contractions produced in the presence of PSB36 10-8M. The effect of PSB36 10-8M caused a rightward shift compared to the curve representing the effect of adenosine in the presence of histamine (Figure 3b). Electrically field stimulated ileum in the presence of the selective adenosine receptor competitive antagonist SCH58261 produced smaller responses (Figure 4a). Following administration of cumulative concentrations the responses decreased however produced no right shift in the dose response curve. Histamine excites the tissue causing it to contract and producing a high response, when adenosine is applied in the presence of SCH58261, the responses are inhibited and acetylcholine release is decreased and thus there is a decrease in the shape of the curve in Figure 4b. The results expressed in Figure 5a illustrate the inhibitory effects of adenosine in the ileum. Figure 5b illustrates the effects of histamine concentrations on the ileum. The experiment was carried out to investigate the best concentration to use so that a maximum and strong, reproducible contraction would be produced; the figure confirmed 110-6M final bath concentration to give the highest and steady contraction of the tissue. Moreover this permitted to obtain a dose-response curve for adenosine with histamine providing a suitable starting concentration for each drug at 110-8M. DISCUSSION The results of this present study show that adenosine plays an inhibitory role on muscular contractility in guinea-pig ileum. Adenosine prevents the neuroeffector transmission in guinea-pig ileum. The action of adenosine appeared to be cholinergic prejunctional in nature, this is portrayed when adenosine is applied to electrically field stimulated guinea-pig ileum(Gustafsson et al., 1985b). The action of adenosine on histamine stimulated guinea-pig ileum in the absence and presence of adenosine antagonists also indicates reduction in neuroeffector transmission however due to postjunctional action. The effect of adenosine on the guinea-pig ileum can be observed in Figure 2. Adenosine produced a dose-dependent depression on the response. It reduced the electrically evoked acetylcholine release from the ileum. A1 and A2 receptors have been reported to reduce acetylcholine release in the gastrointestinal tract(Tomaru et al., 1995). Adenosine released from neuronal endings is thought to have direct actions on smooth muscle as they illustrate relaxant neurotransmitters in the gastrointestinal tract(Storr et al., 2002). Atropine is a competitive antagonist for the muscarinic acetylcholine receptor, consequently when applied to the ileum at 10-6M final bath concentration; there is rapid inhibition of response confirming that the twitch responses were produced by cholinergic nerve stimulation. Adenosine inhibited the twitch response of the electrically stimulated guinea-pig ileum preparation, in the presence of PSB36 10-8M final bath concentration there was a right shift in the adenosine curve thus interpreting that higher concentrations were required to lower the twitch response. The general trends that Figure 3a displays is that the curves have the same form; the linear proportions of the curves are parallel. The traces help to show the changes in the response curve to adenosine and adenosine selective receptor antagonist PSB36. There is a slight fall in tension when adenosine 310-7M was applied in the presence of PSB36 10-8M; however there was a rapid decrease when adenosine 110-5M was administered causing the right shift in the Figure. Upon cumulative additions of adenosine to field stimulated guinea pig ileum the concentration required to inhibit acetylcholine release was of 310-7M, the effects diminish once concentration of adenosine 110-5M was added providing evidence that adenosine has the ability to relax smooth muscle in the ileum. Since PSB36 is a potent and selective A1 adenosine receptor antagonist, the A2A receptors increase electrically induced twitch contractions in the guinea pig ileum, which contributes to assistance of acetylcholine release (Storr et al., 2002). The effects of adenosine in the presence of PSB36 10-7M 10-8M to histamine stimulated guinea pig ileum can be observed in Figure 3b. Histamine administration to the guinea pig ileum caused a tonic histamine contraction which was followed by after-relaxation response, and application of adenosine inhibited the acetylcholine release. The ileum responses were reduced significantly with lower concentrations of adenosine in the presence of PSB36 10-7M and 10-8M compared to the concentrations of adenosine required in the electrically field stimulated ileum confirming that A1 receptors are the subtype present in the guinea pig ileum which cause the inhibition of acetylcholine release. SCH58261 did not significantly affect the position of the rightward shift however additions of cumulative adenosine concentrations caused reduction in the tension produced by the ileum. Large standard error bars can be observed in the figures, these may be due to human handling errors, i.e. micropipetting errors, administrating less or more concentration of adenosine or antagonists. Protein build up causing contamination in organ baths can also contribute to acquiring inaccurate results. Particular cells of the tissue may have become inactive at that moment of time. Furthermore it could be that the piece of thread holding the tissue may have become loose i.e. equipment errors and consequently tension was not measured accurately. It could also be due to unknown errors. Evidence that adenosine inhibits cholinergic neuroeffector transmission in the ileum by a prejunctional action on acetylcholine release can be of functional importance as adenine compounds are released during stimulation of intestinal nerves (Tomaru et al., 1995). Antagonists selective for adenosine receptors are beneficial in the research treatment of numerous conditions including cardiovascular, neurodegenerative and inflammatory diseases. In summary, the present study has confirmed the existence of presynaptic A1 receptors on the parasympathetic nerve terminals in the guinea pig ileum which upon activation causes inhibition of electrically induced neurogenic, cholinergic twitch contractions.

Cuando Cae La Noche :: essays research papers

" De quà © hablamos cuando hablamos de amor ", de Raymond Carver, 157 pà ¡ginas, Editorial Anagrama. Cuando cae la noche. " Todas las narraciones, sean narraciones de hechos reales o de hechos imaginarios, parten de una estructura bà ¡sica, de una estructutura profunda, de puesta en intriga, que algunos llaman fà ¡bula, que presenta una normalidad o equilibrio que se ve alterado, lo cual da inicio a una crisis, el desarrollo de la crisis y la restitucià ³n de ese equlibrio ".Al leer el libro de Carver " De quà © hablamos cuando hablamos de amor ", tal vez el lector se cuestione si estas afirmaciones son correctas.Los cuentos de Carver, podrà ­an considerarse como una excepcià ³n a lo anteriormente dicho, ya que algunos comienzan con una crisis, otros presentan una normalidad, una crisis y el desarrollo de esa crisis, pero no una restitucià ³n de el equilibrio; por eso al leer Carver, el lector debe considerar que no se va a encontrar con la narrativa normal, a la que tal vez, està © acostumbrado. En el cuento que da titulo al libro " De que hablamos...", se nos presenta a dos parejas que discuten sobre lo que es el amor para cada uno de ellos.Los personajes principales son: Mel McGinnis, su segunda esposa Teresa ( a la que llamaban Terri ), Nick ( el narrador de la historia ) y su esposa Laura; Mel es cardià ³logo y Laura es secretaria juridica.El espacio fà ­sico donde se va a desarrollar la accià ³n, es la cocina de la casa de Mel; este es un marco fijo que no posee transformaciones a lo largo de la historia. Uno de los aspectos mà ¡s interesantes que se pueden encontrar en el cuento es el juego de luces que realiza el autor y que reflejan el estado de à ¡nimo de los personajes.Al comenzar la historia Nick ( el narrador ), dice: " El sol, que entraba por el ventanal de detrà ¡s del fregadero, inundaba la cocina.".Esto da al lector una sensacià ³n de luminosidad y se le presenta la conversacià ³n como "normal".Ahora bien, a medida que el relato avanza y el alcohol circula, la noche va envolviendo todo con su negro manto y la conversacià ³n se va tornando cada vez mà ¡s sombrà ­a y saca a relucir otros aspectos de los personajes como la depresià ³n y el alcoholismo. Aunque no todas la historias de Carver son en primera persona la mayorà ­a de ellas si lo son.Este uso de la primera persona permite a Carver hacer emerger la importancia de la percepcià ³n individual.